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- Title
An efficient method for gene knock-down by RNA interference in human skin mast cells.
- Authors
Hazzan, Tarek; Guhl, Sven; Artuc, Metin; Franke, Kristin; Worm, Margitta; Zuberbier, Torsten; Babina, Magda
- Abstract
Mast cells ( MCs) from human skin have been notoriously resistant to gene manipulation, and a method to knock-down gene expression in in situ differentiated MCs is highly desired. The Dharmacon Accell® transfection system proved successful on several 'difficult-to-transfect' cells. In the present work, we therefore tested this method on skin-derived MCs using different si RNA entities. The si RNA was readily taken up, followed by pronounced, specific reduction of gene and protein expression. Hence, we present the first efficient technique for the manipulation of gene expression in primary skin MCs ex vivo, which combines high transfection rates with retained cell viability.
- Subjects
RNA interference; MAST cells; HUMAN genetics; SKIN; PROTEIN expression; GENE expression; ANATOMY
- Publication
Experimental Dermatology, 2017, Vol 26, Issue 11, p1136
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/exd.13358