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- Title
A mi R169 isoform regulates specific NF- YA targets and root architecture in Arabidopsis.
- Authors
Sorin, Céline; Declerck, Marie; Christ, Aurélie; Blein, Thomas; Ma, Linnan; Lelandais‐Brière, Christine; Njo, Maria Fransiska; Beeckman, Tom; Crespi, Martin; Hartmann, Caroline
- Abstract
In plants, roots are essential for water and nutrient acquisition. Micro RNAs (mi RNAs) regulate their target m RNAs by transcript cleavage and/or inhibition of protein translation and are known as major post-transcriptional regulators of various developmental pathways and stress responses. In Arabidopsis thaliana, four isoforms of mi R169 are encoded by 14 different genes and target diverse m RNAs, encoding subunits A of the NF- Y transcription factor complex. These mi RNA isoforms and their targets have previously been linked to nutrient signalling in plants., By using mimicry constructs against different isoforms of mi R169 and mi R-resistant versions of NF- YA genes we analysed the role of specific mi R169 isoforms in root growth and branching., We identified a regulatory node involving the particular mi R169defg isoform and NF- YA2 and NF- YA10 genes that acts in the control of primary root growth. The specific expression of MIM169defg constructs altered specific cell type numbers and dimensions in the root meristem. Preventing mi R169defg-regulation of NF- YA2 indirectly affected laterial root initiation. We also showed that the mi R169defg isoform affects NF- YA 2 transcripts both at m RNA stability and translation levels., We propose that a specific mi R169 isoform and the NF- YA2 target control root architecture in Arabidopsis.
- Subjects
ARABIDOPSIS; MICRORNA; MESSENGER RNA; TRANSCRIPTION factors; MERISTEMS; PLANT growth
- Publication
New Phytologist, 2014, Vol 202, Issue 4, p1197
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.12735