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- Title
Magnoliae Flos Essential Oil as an Immunosuppressant in Dendritic Cell Activation and Contact Hypersensitivity Responses.
- Authors
Chen, Chun-Hsien; Chen, Hsin-Chun; Chang, Wen-Te; Lee, Meng-Shiou; Liu, Yi-Chen; Lin, Ming-Kuem
- Abstract
Magnoliae Flos is a commonly used traditional medicinal material in Asia. It is used to treat sinusitis, nasal congestion, and hypersensitive skin. Because Magonlia Flos was described as an aromatic material in ancient Chinese texts, we hypothesized that its essential oil may be used to treat immune disorders. Dendritic cells (DCs), regarded as a major target of immunomodulators to control immune responses, play a critical role in the adaptive immune response. In this study, Magnoliae Flos essential oil (MFEO) decreased the production of the cytokines TNF- α , IL-6, and IL-12p70 in lipopolysaccharide (LPS)-stimulated DCs. It also suppressed the surface markers MHC II, CD80, and CD86 in LPS-stimulated DCs. Animal models demonstrated that the 2,4-Dinitro-1-fluorobenzene (DNFB) inducing a contact hypersensitivity response was inhibited following treatment with MFEO. In addition, MFEO inhibited the infiltration of T cells in the ears of DNFB-induced mice. To explore its bioactive compounds, the components of MFEO were analyzed using gas chromatography (GC) and GC-mass spectrometry. The results revealed that the major compounds in MFEO are camphor and 1,8-cineole. Additional DC bioassays confirmed that these compounds substantially suppressed cytokine production in LPS-induced DCs. Therefore, we demonstrated that MFEO exhibits an immunosuppressive effect both in vivo and in vitro, and camphor and 1,8-cineole may be the major components responsible for its immunosuppressive ability. The findings indicate that MFEO has the potential to be developed as a new immunosuppressant for excessive diseases.
- Subjects
TAIWAN; ANIMAL experimentation; ANTIGENS; APOPTOSIS; BIOLOGICAL assay; CONTACT dermatitis; CYTOKINES; DENDRITIC cells; ESSENTIAL oils; GAS chromatography; IMMUNOSUPPRESSIVE agents; INTERLEUKINS; MASS spectrometry; CHINESE medicine; MICE; RESEARCH funding; TUMOR necrosis factors; TREATMENT effectiveness; LIPOPOLYSACCHARIDES; IN vitro studies; IN vivo studies
- Publication
American Journal of Chinese Medicine, 2020, Vol 48, Issue 3, p597
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X20500305