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- Title
Water-Soluble Tetraphenylethene Derivatives as Fluorescent 'Light-Up' Probes for Nucleic Acid Detection and Their Applications in Cell Imaging.
- Authors
Hong, Yuning; Chen, Sijie; Leung, Chris Wai Tung; Lam, Jacky Wing Yip; Tang, Ben Zhong
- Abstract
The detection of , such as DNA and RNA, plays a significant role in , forensics, and bioinformatics. Traditional nucleic acid probes are mainly intercalators, which are potential mutagens, or groove binders that show high preference only for double-stranded DNA. We herein present two versatile fluorescent probes for nucleic acid detection and visualization. The nonemissive tetraphenylethene derivatives (TTAPE) are induced by DNA/RNA to emit, thereby showing a novel phenomenon of aggregation-induced emission (AIE). This kind of 'light-up' property enables the quantitation and visualization of in aqueous solution and electrophoretic gels, respectively. The cationic TTAPE can penetrate cells with a compromised plasma membrane easily but cannot enter live cells with an intact membrane, thus making them useful for the differentiation between dead and live cells. On account of the high binding affinity to DNA, TTAPE can selectively label the chromosomes and nuclei in fixed cells, which provides a simple and fast method for the observation of cell mitosis. Owing to their AIE characteristics, the dye molecules aggregate in DNA-rich regions and exert appreciable quantum efficiency as well as superior photostability.
- Subjects
NUCLEIC acid probes; CLUSTERING of particles; ELECTROSTATIC interaction; FLUORESCENT probes; NUCLEIC acids; PHENYLENE compounds
- Publication
Chemistry - An Asian Journal, 2013, Vol 8, Issue 8, p1806
- ISSN
1861-4728
- Publication type
Article
- DOI
10.1002/asia.201300065