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- Title
PCR BASED DETECTION OF SUBCLINICAL BOVINE BABESIOSIS IN PUNJAB.
- Authors
Singh, Harkirat; Jyoti, M. Haque; Singh, N. K.; Rath, S. S.
- Abstract
A PCR based assay was standardized for the specific and sensitive detection of Babesia bigemina infection in bovine blood samples. Among the 104 samples collected from apparently healthy carrier cattle, an amplicon of 278 bp size specific for B. bigemina was detected in 25.96% animals against the routine blood smear examination, which revealed parasitic piroplasms in only 3.84% of samples. The use of PCR resulted in significantly higher efficacy of detection of the parasite compared to microscopic examination of blood smears. These results clearly demonstrate that bovine babesiosis is common and endemic in the state and PCR is the optimal approach for the detection of subclinical cases.
- Subjects
PUNJAB (India); BABESIOSIS; BABESIA bigemina; CATTLE; POLYMERASE chain reaction
- Publication
Indian Journal of Animal Research, 2013, Vol 47, Issue 6, p543
- ISSN
0367-6722
- Publication type
Article