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- Title
EI of the Phosphotransferase System of Escherichia coli :Mathematical Modeling Approach to Analysis of Its Kinetic Properties.
- Authors
Karelina, T. A.; Ma, H.; Goryanin, I.; Demin, O. V.
- Abstract
The mathematical model of the operation of the first enzyme of the Escherichia coli phosphotransferase system, EI, is proposed. Parameters of the kinetic model describing the operation of EI under different conditions are identified on the basis of a large amount of known experimental data. The verified model is employed to predict modes of operation of EI under both in vivo physiological conditions and in vitro nonphysiological conditions. The model predicts that under in vivo physiological conditions, the rate of phosphotransfer from EI to the second protein of the phosphotransferase system HPr by the dimer is much higher than by themonomer. A hypothesis is proposed on the basis of calculations that the transfer by amonomer plays a role in the regulation of chemotaxis. At submicromolar pyruvate concentration, the model predicts nonmonotonic dependence of the phosphotransfer rate on the substrate (PEP) concentration.
- Subjects
PHOSPHOTRANSFERASES; ESCHERICHIA coli; MATHEMATICAL models; DIMERS; CHEMOTAXIS; PYRUVATES
- Publication
Journal of Biophysics, 2011, p1
- ISSN
1687-8000
- Publication type
Article
- DOI
10.1155/2011/579402