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- Title
CPNE1 is a target of miR-335-5p and plays an important role in the pathogenesis of non-small cell lung cancer.
- Authors
Tang, Haicheng; Zhu, Jianjie; Du, Wenwen; Liu, Shunlin; Zeng, Yuanyuan; Ding, Zongli; Zhang, Yang; Wang, Xueting; Liu, Zeyi; Huang, Jianan
- Abstract
Background: Despite advances in diagnosis and treatment, the survival of non-small cell lung cancer (NSCLC) patients remains poor. There is therefore a strong need to identify potential molecular targets for the treatment of NSCLC. In the present study, we investigated the function of CPNE1 in the regulation of cell growth, migration and invasion. Methods: Quantitative real-time PCR (qRT-PCR) was used to detect the expression of <italic>CPNE1</italic> and miR-335-5p. Western blot and immunohistochemical assays were used to investigate the levels of CPNE1 and other proteins. Flow cytometry was used to determine cell cycle stage and apoptosis. CCK-8 and clonogenic assays were used to investigate cell proliferation. Wound healing, migration and invasion assays were used to investigate the motility of cells. A lung carcinoma xenograft mouse model was used to investigate the in vivo effects of CPNE1 overexpression. Results: We observed that knockdown of CPNE1 and increased expression of miR-335-5p inhibits cell proliferation and motility in NSCLC cells, and found that CPNE1 was a target of miR-335-5p. In addition, our data indicated that CPNE1 inhibition could improve the clinical effects of EGFR-tyrosine kinase inhibitors. Conclusions: The present results indicate that CPNE1 may be a promising molecular target in the treatment of NSCLC.
- Subjects
MICRORNA; CARCINOGENESIS; FLOW cytometry; POLYMERASE chain reaction; NON-small-cell lung carcinoma
- Publication
Journal of Experimental & Clinical Cancer Research (17569966), 2018, Vol 37, Issue 1, pN.PAG
- ISSN
1756-9966
- Publication type
Article
- DOI
10.1186/s13046-018-0811-6