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- Title
Effects of root exudates of clover (Trifolium repens) on PAH microbial degradation and dioxygenase.
- Authors
WANG Yue; GUO Mei-xia; JIN Jing-hua; GONG Zong-qiang; JIA Chun-yun; LI Xiao-jun; ZHANG Wei
- Abstract
To demonstrate rhizospheric effect on the mechanism of (polycyclic aromatic hydrocar- bon) PAH degradation, and to establish a proper joint phyto-microbial remediation mode, micro- cosms containing microorganisms and PAHs (pyrene and benzo[a]Pyrene) were added with clover (Trifolium repens) root exudates to study their effects on PAH degradation. Dioxygenase gene and 16S rDNA gene copy number changes during the biodegradation process were analyzed, and the microorganism with a good ability for degrading PAHs was identified. The results showed that Myco- bacterium M1 had the capability to degrade PAHs. When total organic carbon (TOC) concentration of clover root exudates was 35.5 mg · L-1, pyrene and benzo[a]pyrene degradation rates increased significantly, and the proportion of dioxygenase gene to 16S rDNA of Mycobacterium M1 increased. In the biodegradation process, dioxygenase gene copy number increased significantly, whereas 16S rDNA copy number increase was not so obvious, showing that the former was related to degradation process, but the latter was related to microbial numbers. It was concluded that the clover root exu- dates promoted the dioxygenase gene copy number of Mycobacterium M1, which contributed to the degradation of PAHs.
- Publication
Chinese Journal of Applied Ecology / Yingyong Shengtai Xuebao, 2014, Vol 25, Issue 11, p3145
- ISSN
1001-9332
- Publication type
Article