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- Title
Micronuclei as prognostic marker.
- Authors
Sherashiya, Pinhaja; Yogesh T. L.; Hemavathy S.; Nonitha S.; Aaliya K.; Pallavi K.
- Abstract
Background: Micronucleus is a microscopically visible round or oval cytoplasmic chromatin mass in the extranuclear vicinity, originating from aberrant mitosis. Oral habits of smoking tobacco damage the oral tissues. An assessment of the damage is feasible by the presence and number of micronuclei in different site of oral cavity exfoliated cells of the oral tissues using smears. Aims: The present study design is to assess the micronuclei number in different site of oral cavity (buccal mucosa, floor of the mouth and labial mucosa) to assess the genotoxic damage in smokers, nonsmokers and in the persons who had quit the habit recently. Materials and Methods: Three groups (smokers, recently quit habit and non-tobacco habit) of 15 individuals each were clinically examined. Different site of oral cavity (buccal mucosa, floor of the mouth and labial mucosa) cytosmears was taken by using ice cream stick spatula and stained with Papanicolaou (PAP) stain. Presence of micronuclei will be assessed under x40 magnification in polarized light microscope and counted. Results: The study showed that smears of individuals with smoking habits showed higher number of micronuclei count seen in buccal mucosa than labial mucosa and floor of the mouth. Our study showed P ≤ 0.00, which is highly significant. The number of micronuclei was greater in patients who recently quit habit than non-tobacco habit individuals. Conclusion: The genotoxic effects of tobacco smoke cause micronuclei and count can be used as a noninvasive early detection tool. This observation is vital in utilization of the micronuclei detection in different sites of oral cavity and prognosis on quitting the habit.
- Subjects
NUCLEOLUS; ORAL diseases; PHYSIOLOGICAL effects of tobacco
- Publication
Journal of Oral & Maxillofacial Pathology (0973029X), 2014, Vol 18, pS39
- ISSN
0973-029X
- Publication type
Abstract