We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A role for the POU-III transcription factor Brn-4 in the regulation of striatal neuron precursor differentiation.
- Authors
Shimazaki, Takuya; Arsenijevic, Yvan; Ryan, Aimee K.; Rosenfeld, Michael G.; Weiss, Samuel
- Abstract
Both insulin-like growth factor-I (IGF-I) and brain-derived neurotrophic factor (BDNF) induce the differentiation of post-mitotic neuronal precursors, derived from embryonic day 14 (E14) mouse striatal multipotent stem cells. Here we ask whether this differentiation is mediated by a member of the POU-III class of neural transcription factors. Exposure of stem cell progeny to either IGF-I or BDNF resulted in a rapid upregulation of Brn-4 mRNA and protein. Indirect immunocytochemistry with Brn-4 antiserum showed that the protein was expressed in newly generated neurons. Other POU-III genes, such as Brn-1 and Brn-2, did not exhibit this upregulation. Basic FGF, a mitogen for these neuronal precursors, did not stimulate Brn-4 expression. In the E14 mouse striatum, Brn-4-immunoreactive cells formed a boundary between the nestin-immunoreactive cells of the ventricular zone and the β-tubulin-immunoreactive neurons migrating into the mantle zone. Loss of Brn-4 function during the differentiation of stem cell-derived or primary E14 striatal neuron precursors, by inclusion of antisense oligonucleotides, caused a reduction in the number of β-tubulin-immunoreactive neurons. These findings suggest that Brn-4 mediates, at least in part, the actions of epigenetic signals that induce striatal neuron-precursor differentiation.
- Subjects
TRANSCRIPTION factors; NEURONS; CELL differentiation; STEM cells; PROTEINS; IMMUNOCYTOCHEMISTRY; DEVELOPMENTAL neurobiology
- Publication
EMBO Journal, 1999, Vol 18, Issue 2, p444
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/18.2.444