We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Identification of an S-adenosylhomocysteine hydrolase-like transcript induced during dendritic cell differentiation.
- Authors
Dekker, James W.; Budhia, Sangeeta; Angel, Nicola Z.; Cooper, Benjamine J.; Clark, Georgina J.; Hart, Derek N. J.; Kato, Masato
- Abstract
Dendritic cells (DC) are the professional antigen-presenting cells that initiate immune responses. While DC take up antigen, migrate to lymph nodes and present processed antigen to T lymphocytes, little is known of the intracellular biochemical pathways controlling these events. Using the differential display technique, employing the activated blood DC-like cell line L428, we isolated a cDNA induced during DC differentiation likely to have a regulatory function. This cDNA encoded a putative 530-amino-acid (aa) protein consisting of a unique hydrophilic domain (106 aa) and a domain (424 aa) similar to the methylation pathway enzyme S-adenosylhomocysteine hydrolase (AHCY). Therefore, this molecule was termed DC-expressed AHCY-like molecule (DCAL). DCAL mRNA was expressed moderately in fresh blood DC, but was not detectable in other peripheral blood mononuclear cells. DCAL mRNA increased markedly during activation of blood and skin DC (Langerhans cells), but was diminished in terminally differentiated tonsil DC. Cultured monocytes expressed little DCAL mRNA, but levels increased markedly when differentiated into DC by cytokines GM-CSF and IL-4. The DCAL gene [Chromosome (Chr) 1] and another previously identified DCAL-like molecule KIAA0828 (Chr 7) differed from the AHCY gene (Chr 20) in gene organization. Thus, DCAL may have a role in controlling critical events in DC differentiation and belong to a novel family of AHCY-like molecules.
- Subjects
DENDRITIC cells; ANTIGEN presenting cells; HOMOCYSTEINE; SULFUR amino acids; HYDROLASES; CELL differentiation; IMMUNE response; GENETICS
- Publication
Immunogenetics, 2002, Vol 53, Issue 12, p993
- ISSN
0093-7711
- Publication type
Article
- DOI
10.1007/s00251-001-0402-z