We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Oxidative stress damage and oxidative stress responses in the choroid plexus in Alzheimer’s disease.
- Authors
Perez-Gracia, Ester; Blanco, Rosa; Carmona, Margarita; Carro, Eva; Ferrer, Isidro
- Abstract
Choroid plexus homogenates from 27 cases with Alzheimer disease-related pathology (AD), stages I/0 ( n = 5), III–IV/0-B ( n = 15) and V–VI/B-C ( n = 7) and 3 age-matched controls (no clinical symptoms, no neuropathological lesions) were processed for gel electrophoresis and western blotting for oxidation markers carboxymethyl-lysine (CML) and N-carboxyethyl-lysine (CEL). Increased CEL and CML expression was seen in AD cases stages IVB, and V–VI/B-C when compared to controls and cases with AD-related pathology classified as I/0 and III/0. Variable stress damage was seen in stage III/B. Although lower stages of AD did not show β-amyloid deposition in the choroid plexus, the amount of β-amyloid was very variable at stages V/VI as revealed by western blotting, suggesting that other factors in addition to local fibrillar β-amyloid were associated with oxidative damage in the choroid plexus. Two-dimensional gel electrophoresis and western blotting to CEL and CML in combination with mass spectrometry disclosed increased intensity of variable spots in AD cases leading to the identification of tyrosine 3/tryptophan 5-monooxygenase activation protein, zeta polypeptide, tropomyosin 3 isoform, and apolipoprotein A-II (ApoA-II) as targets of increased oxidative damage in AD. Oxidation of these proteins may result in impaired protein interactions, protein folding and protein kinase activity; abnormal function of endothelial and vascular smooth muscle cells; and impaired HDL-cholesterol metabolism in the choroid plexus in advanced stages of AD.
- Subjects
ALZHEIMER'S disease; OXIDATIVE stress; CHOROID plexus; PHYSIOLOGICAL stress; PREVENTIVE medicine; ISOPENTENOIDS
- Publication
Acta Neuropathologica, 2009, Vol 118, Issue 4, p497
- ISSN
0001-6322
- Publication type
Article
- DOI
10.1007/s00401-009-0574-4