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- Title
Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized <sup>13</sup>C Magnetic Resonance Spectroscopic Imaging.
- Authors
Butt, Sadia A; Søgaard, Lise V; Magnusson, Peter O; Lauritzen, Mette H; Laustsen, Christoffer; Åkeson, Per; Ardenkjær-Larsen, Jan H
- Abstract
The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-13C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-13C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.
- Subjects
BRAIN metabolism; MAGNETIC resonance imaging of the brain; SPECTROSCOPIC imaging; AMINOTRANSFERASES; GLUTAMIC acid metabolism; NEURODEGENERATION
- Publication
Journal of Cerebral Blood Flow & Metabolism, 2012, Vol 32, Issue 8, p1508
- ISSN
0271-678X
- Publication type
Article
- DOI
10.1038/jcbfm.2012.34