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- Title
Alternative use of phage display: phage M13 can remain viable in the intestines of poultry without causing damage.
- Authors
de Almeida Araújo Santos, Fabiana; Valadares Junior, Edson Campos; Goulart, Luiz Ricardo; Nunes, Pedro Lucas Figueiredo; Mendonça, Eliane Pereira; Girão, Lúcio Vilela Carneiro; da Hora, Aline Santana; Ferreira, Thatiana Bragine; Bastos, Luciana Machado; Medeiros-Ronchi, Alessandra Aparecida; Fonseca, Belchiolina Beatriz
- Abstract
Phage display (PD) is a tool for developing new molecules to control pathogens. Peptides selected by PD are commonly synthesised and tested, but the use of phage M13 displaying the selected peptides as a direct biding in the intestinal tract has not yet been tested. This study evaluated whether phage M13 can remain viable in the chicken gastrointestinal tract and whether it causes injury or humoral immune response. We inoculated phage M13 or E. coli ER2738 (ECR) infected with M13 into birds at different ages. We found the virus in faeces at 5 or 13 days after inoculation, just when it infected the ECR. The presence of phage M13 or ECR did not result in gut injuries and had no impacts on weight gain and bird health. Furthermore, the levels of IgY were similar in all treatments, which indicates that the virus can be used in chicken until 42 days without being recognised by the immune system. This work provides a scientific basis for the use of PD as a tool in numerous applications to control different pathogens. Key Points: Phage M13 remains viable in the bird's intestine if inoculated with E. coli ER2738. Bacteriophage M13 does not damage the chicken gut. Phage M13 remains in the gut without leading to a humoral response up to 42 days.
- Subjects
ESCHERICHIA coli; BACTERIOPHAGES; HUMORAL immunity; INTESTINES; POULTRY
- Publication
AMB Express, 2022, Vol 12, Issue 1, p1
- ISSN
2191-0855
- Publication type
Article
- DOI
10.1186/s13568-022-01407-9