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- Title
Junctional recruitment of mammalian Scribble relies on E-cadherin engagement.
- Authors
Navarro, Christel; Nola, Sébastien; Audebert, Stéphane; Santoni, Marie-Josée; Arsanto, Jean-Pierre; Ginestier, Christophe; Marchetto, Sylvie; Jacquemier, Jocelyne; Isnardon, Daniel; Le Bivic, André; Birnbaum, Daniel; Borg, Jean-Paul
- Abstract
Members of the LAP protein family, LET-413 in Caenorhabditis elegans, Scribble in Drosophila melanogaster, and Erbin, Lano, Densin-180 and hScrib in mammals, have conserved structural features. LET-413 and Scribble are junctional proteins involved in establishing and maintaining epithelial cell polarity. scribble also behaves as a neoplastic tumor suppressor gene. We show here that, in epithelial cells, hScrib is recruited at cell–cell junctions in an E-cadherin-dependent manner as shown by calcium switch assays in MDCK cells, re-expression of E-cadherin in MDA-231 cells treated by 5-Aza-2′-deoxycytidine (5Aza), and siRNA experiments. hScrib is restricted at the basolateral membrane of epithelial cells by its LRR domain, and is enriched in Triton X-100-insoluble fractions. In breast cancers, most lobular tumors did not express hScrib and E-cadherin while ductal tumors had a less frequent downregulation of hScrib. Our data provide additional insights on the modalities of recruitment of hScrib at the cell–cell junctions, and establish a potential link between the E-cadherin and hScrib tumor suppressors.Oncogene (2005) 24, 4330–4339. doi:10.1038/sj.onc.1208632 Published online 4 April 2005
- Subjects
CADHERINS; BREAST cancer; CELL junctions; CELL adhesion molecules; TUMORS; MEMBRANE fusion
- Publication
Oncogene, 2005, Vol 24, Issue 27, p4330
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1208632