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- Title
Aerobic exercise inhibits acute lung injury: from mouse to human evidence Exercise reduced lung injury markers in mouse and in cells.
- Authors
Rigonato-Oliveira, Nicole Cristine; MacKenzie, BreAnne; Lacerda Bachi, Andre Luis; Oliveira-Junior, Manoel Carneiro; Santos-Dias, Alana; Brandao-Rangel, Maysa Alves Rodrigues; Delle, Humberto; Costa-Guimaraes, Tamara; Damaceno-Rodrigues, Nilsa Regina; Hilário Dulley, Larissa; Anhesini Benetti, Marcela; Malfitano, Christiane; de Angelis, Kátia; Albertini, Regiane; Ligeiro Oliveira, Ana Paula; Abbasi, Asghar; Northoff, Hinnak; Paula Vieira, Rodolfo
- Abstract
Acute respiratory distress syndrome (ARDS) is defined as hypoxemic respiratory failure with intense pulmonary inflammation, involving hyperactivation of endothelial cells and neutrophils. Given the anti-inflammatory effects of aerobic exercise (AE), this study investigated whether AE performed daily for 5 weeks would inhibit extra-pulmonary LPS-induced ARDS. C57Bl/6 mice were distributed into Control, Exercise, LPS and Exercise+LPS groups. AE was performed on a treadmill for 5x/week for four weeks before LPS administration. 24hours after the final AE physical test, animals received 100ug of LPS intra-peritoneally. In addition, whole blood cell culture, neutrophils and human endothelial cells were preincubated with IL-10, an anti-inflammatory cytokine induced by exercise. AE reduced total protein levels (p<0.01) and neutrophil accumulation in bronchoalveolar lavage (BAL) (p<0.01) and lung parenchyma (p<0.01). AE reduced BAL inflammatory cytokines IL-1β, IL-6 and GM-CSF (p<0.001), CXCL1/KC, IL-17, TNF-alpha and IGF-1 (p<0.01). Systemically Systemically, AE reduced IL-1β, IL-6 and IFN-gamma (p<0.001), CXCL1/KC (p<0.01) and TNF-alpha (p<0.05). AE increased IL-10 levels in serum (p<0.001) and BAL (p<0.001). Furthermore, AE increased superoxide dismutase SOD (p<0.01) and decreased superoxide anion accumulation in the lungs (p<0.01). Lastly, pre-incubation with IL-10 significantly reduced LPS-induced activation of whole blood cells, neutrophils and HUVECs, as observed by reduced production of IL-1β, IL-6, IL-8 and TNF-alpha. Our data suggest that AE inhibited LPS-induced lung inflammation by attenuating inflammatory cytokines and oxidative stress markers in mice and human cell culture via enhanced IL-10 production.
- Subjects
AEROBIC exercises; LUNG injury prevention; ADULT respiratory distress syndrome; ENDOTHELIAL cells; NEUTROPHILS
- Publication
Exercise Immunology Review, 2018, Vol 24, p48
- ISSN
1077-5552
- Publication type
Article