We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The NtSPL Gene Family in Nicotiana tabacum : Genome-Wide Investigation and Expression Analysis in Response to Cadmium Stress.
- Authors
He, Linshen; Peng, Xiang; Cao, Hanping; Yang, Kunjian; Xiang, Lien; Li, Rui; Zhang, Fangyuan; Liu, Wanhong
- Abstract
The SQUAMOSA promoter binding protein-like (SPL)SPL family genes play an important role in regulating plant growth and development, synthesis of secondary metabolites, and resistance to stress. Understanding of the role of the SPL family in tobacco is still limited. In this study, 42 NtSPL genes were identified from the genome of the tobacco variety TN90. According to the results of the conserved motif and phylogenetic tree, the NtSPL genes were divided into eight subgroups, and the genes in the same subgroup showed similar gene structures and conserved domains. The cis-acting element analysis of the NtSPL promoters showed that the NtSPL genes were regulated by plant hormones and stresses. Twenty-eight of the 42 NtSPL genes can be targeted by miR156. Transcriptome data and qPCR results indicated that the expression pattern of miR156-targeted NtSPL genes was usually tissue specific. The expression level of miR156 in tobacco was induced by Cd stress, and the expression pattern of NtSPL4a showed a significant negative correlation with that of miR156. These results suggest that miR156-NtSPL4a may mediate the tobacco response to Cd stress. This study lays a foundation for further research on the function of the NtSPL gene and provides new insights into the involvement of NtSPL genes in the plant response to heavy metal stress.
- Subjects
GENE expression; GENE families; PLANT genes; CADMIUM; TOBACCO; PLANT hormones
- Publication
Genes, 2023, Vol 14, Issue 1, p183
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes14010183