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- Title
Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste.
- Authors
Sepahy, Abbas Akhavan; Ghazi, Shokoofeh; Sepahy, Maryam Akhavan
- Abstract
A xylanase producer Bacillus mojavensis strain, called AG137, isolated from cotton farm (Kashan-Iran). The optimal xylanase activity reached at 55°C & pH 9.0. Enzyme yield was studied using a medium with different agricultural wastes as inducers. Xylanase production of about 249.308 IU/mL was achieved at pH 8 and 37°C, within 48 h submerged fermentation in enzyme production medium supplemented with 2% (w/v) oat bran as an optimum carbon source. A mixture of 1% (w/v) yeast extract and 1%(w/v) tryptone as optimum nitrogen sources, agitation speed 200 rpm, and inoculumsize 2%(v/v) were the optimums for maximum production. Accordingly, xylanase yield from 194.68 IU/mL under non-optimized fermentation condition enhanced to 302.466 IU/mL in optimized condition. Screened xylanase is thermostable, presenting 70% stability at 60?C during 30 min. Further enzyme incubation in higher temperature caused a decrease in the residual enzyme activity, yet it retained 68%-50% of its activity after 1 hour from 45?C to 55°C. Besides, it is stable in pH 9 and 10, maintaining over 70% of its activity for 2 h. The enzyme also could preserve 71% and 63% of its initial activity after 3 hours of pre-incubation in the same alkaline condition. Produced xylanase therefore was introduced as an alkaline-active and stable one, displaying suitable thermostability feature, confirmed by HPLC analysis. Hence, all xylanase properties highlight its promising uses in industrial scale.
- Subjects
KASHAN (Iran); IRAN; XYLANASES; BIOCONVERSION; BACILLUS (Bacteria); INDUSTRIAL wastes; MICROBIOLOGY
- Publication
Enzyme Research, 2011, p1
- ISSN
2090-0406
- Publication type
Article
- DOI
10.4061/2011/593624