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- Title
Micro RNA expression analysis and Multiplex ligation-dependent probe amplification in metastatic and non-metastatic uveal melanoma.
- Authors
Larsen, Ann ‐ Cathrine; Holst, Line; Kaczkowski, Bogumil; Andersen, Morten T.; Manfé, Valentina; Siersma, Volkert D.; Kolko, Miriam; Kiilgaard, Jens F.; Winther, Ole; Prause, Jan U.; Gniadecki, Robert; Heegaard, Steffen
- Abstract
Purpose To determine the association of micro RNA expression and chromosomal changes with metastasis and survival in uveal melanoma ( UM). Methods Thirty-six patients with UM were selected based on the metastatic status, and clinicopathological data were collected. Multiplex ligation-dependent probe amplification ( MLPA) was used to identify chromosomal changes. Chromosomal changes and clinicopathological data were correlated with survival and metastasis. The micro RNA expression was analysed in 26 of the 36 archived UM samples. Unsupervised analysis, differential expression analysis and Cox regression analysis were performed to determine the association with metastasis and survival. Results Metastasis and metastatic death occurred in 20 patients, two patients died of other causes and one patient of unknown causes. A significant association between increasing size category (p = 0.002, log-rank), extraocular extension (p = 0.001), chromosome 3 loss (p = 0.033) and 1p loss (p = 0.030) and development of metastases was observed. Tumour, node, metastasis ( TNM) staging showed a significant association with survival (p < 0.0001, log-rank). Adjusting for gender and age TNM size category T4 (p = 0.016, Cox regression analysis), mixed (p = 0.029) and epithelioid (p = 0.0058) cell types, chromosome 3 loss (p = 0.014) and 8q gain (p = 0.010) were significant prognosticators for a poor survival. Hierarchical clustering divided the UM into three groups based on micro RNA expression. The clusters showed no association with clinical or histopathological features, TNM staging, metastasis or survival. Differential expression analysis did not reveal micro RNAs related to metastasis or survival. Conclusions The prognostic significance of chromosome 3 loss and 8q gain identified by MLPA analysis was confirmed in archived UM samples. The value of micro RNA expression as a predictor of metastasis and survival in UM could not be confirmed.
- Subjects
MESSENGER RNA; GENE expression; MELANOMA; NEUROENDOCRINE tumors; EYE diseases; OPHTHALMOLOGY
- Publication
Acta Ophthalmologica (1755375X), 2014, Vol 92, Issue 6, p541
- ISSN
1755-375X
- Publication type
Article
- DOI
10.1111/aos.12322