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- Title
RNA-electroporated CD40-activated B cells induce functional T-cell responses against HepG2 cells.
- Authors
Shen, S. N.; Xu, Z.; Qian, X. P.; Ding, Y. T.; Yu, L. X.; Liu, B. R.
- Abstract
Hepatocellular carcinoma (HCC) is one of the incurable tumours in the world. Cell-based immunotherapy, in which antigen-loaded antigen-presenting cells (APCs) are able to elicit T cell responses, has become an alternative treatment for liver cancer. Here, we used HepG2 cells′ total RNA-electroporated CD40 ligand-activated B (CD40-B) cells as alternative APC for induction of specific CD8+ T-cell responses. The antigen-presenting ability of CD40-B cells was determined by phenotypic analysis, showing a polyclonal, strongly activated B-cell population with high expression of co-stimulatory molecules. To demonstrate the ability of total RNA extracted from HepG2 cells electroporated CD40-B cells to induce CD8+ T-cell responses, these RNA-loaded cells were co-cultured with autologous peripheral blood mononuclear cells for 7 days followed by analysis of T-cell antigen specificity. These experiments showed that CD40-B cells electroporated with HepG2 cells′ total RNA are capable of activating antigen-specific interferon-γ-producing CD8+ T cells, and these T cells activated by CD40-B cells show a killing effect on HepG2 cells. These findings demonstrated that the carcinoma cell derived total RNA-electroporated CD40-B cells could be used as alternative APC for the induction of antigen-specific CD8+ T-cell responses, which might be used in HCC immunotherapy.
- Subjects
LIVER cancer; CANCER immunotherapy; ANTIGEN presenting cells; ELECTROPORATION; ALTERNATIVE medicine; RNA synthesis; T cells
- Publication
European Journal of Cancer Care, 2008, Vol 17, Issue 4, p404
- ISSN
0961-5423
- Publication type
Article
- DOI
10.1111/j.1365-2354.2007.00841.x