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- Title
Delivery systems of CRISPR/Cas9-based cancer gene therapy.
- Authors
Biagioni, Alessio; Laurenzana, Anna; Margheri, Francesca; Chillà, Anastasia; Fibbi, Gabriella; so, Mario
- Abstract
CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) is today one of the most reliable method for gene-editing, supporting previous gene therapies technologies such as TALEN, Meganucleases and ZFNs. There is a growing up number of manuscripts reporting several successful gene-edited cancer cell lines, but the real challenge is to translate this technique to the clinical practice. While treatments for diseases based on a single gene mutation is closer, being possible to target and repair the mutant allele in a selective way generating specific guide RNAs (gRNAs), many steps need to be done to apply CRISPR to face cancer. In this review, we want to give a general overview to the recent advancements in the delivery systems of the CRISPR/Cas9 machinery in cancer therapy.
- Subjects
CANCER genes; CRISPRS; GENOME editing; GENE therapy; TUMORS
- Publication
Journal of Biological Engineering, 2018, Vol 12, Issue 1, pN.PAG
- ISSN
1754-1611
- Publication type
Article
- DOI
10.1186/s13036-018-0127-2