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- Title
Effect on hypoxia/reoxygenation-induced cardiomyocyte injury and Pink1/Parkin pathway.
- Authors
Xiyang Lu; Guangwei Huang; Hailong Bao; Zonggang Duan; Chao Li; Muzhi Lin; Haiyan Zhou; Zhenhua Luo; Wei Li
- Abstract
Our study aimed to detect the effects of proprotein convertase subtilisin/kexin type 9 (PCSK9) on exacerbating cardiomyocyte hypoxia/reoxygenation (H/R) injury and the possible mechanism. A cell model of H/R was constructed. PCSK9 mRNA and protein levels were significantly upregulated during AC16 cardiomyocyte H/R. Flowmetry detection of apoptosis, as well as JC-1, confirmed that PCSK9 upregulation of autophagy levels was accompanied by apoptosis. Furthermore, in the H/R+si-PCSK9 group, the expression of autophagy-related protein LC3 decreased and P62 increased. At the same time, the presentation of the autophagic pathway Pink1/Parkin was also downregulated. In conclusion, in AC16 cardiomyocytes treated with H/R, PCSK9 expression and autophagy levels were increased; a possible molecular mechanism was the activation of the Pink1/Parkin pathway.
- Subjects
SMALL interfering RNA; PROTEIN expression; SUBTILISINS
- Publication
General Physiology & Biophysics, 2023, Vol 42, Issue 1, p87
- ISSN
0231-5882
- Publication type
Article
- DOI
10.4149/gpb_2022045