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- Title
Platelet membrane abnormality in Alzheimer's disease.
- Authors
Zubenko, George S.; Cohen, Bruce M.; Boller, Francois; Malinakova, Ivana; Keefe, Nanci; Chojnacki, Bonnie
- Abstract
Fluorescence studies of membrane fluidity were conducted double-blind using platelet and red cell membranes prepared from 24 demented patients with probable Alzheimer's disease and 36 neurologically healthy subjects. The fluidity of the hydrocarbon and lipid-aqueous interface regions of cell membranes was determined at 37°C by fluorescence spectroscopy using the lipid probes 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH), respectively. The rotation rate of TMA-DPH in labeled platelet membranes did not differ between the groups. In contrast, the rotation rate of DPH in labeled platelet membranes from the demented patients (2.15 ± 0.24 × 108/sec, SD) was greater than that for the normal controls (1.93 ± 0.13 × 108/sec, SD, p = 3.8 × 10−5). This difference was reflected by a reduction in the steady-state anisotropy of DPH in labeled platelets from the demented group (0.1887 ± 0.0085, SD) when compared to the respective mean for the controls (0.2000 ± 0.0060, SD; p = 1.3 × 10−7). Abnormal membrane fluidity was significantly correlated with severity of dementia, but not with duration of illness or apparent age of onset. The findings do not support the hypothesis that Alzheimer's disease results from a pathological acceleration of the normal aging process, since normal aging is associated with Decemberreased fluidity of cell membranes from platelets, as well as from lymphocytes, hepatocytes, and neurons.
- Publication
Annals of Neurology, 1987, Vol 22, Issue 2, p237
- ISSN
0364-5134
- Publication type
Article
- DOI
10.1002/ana.410220208