We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Changes in vocal fold gene expression and histology after injection augmentation in a recurrent laryngeal nerve injury model.
- Authors
Malka, Ronit; Isaac, Alisa; Gonzales, Gabriela; Miar, Solaleh; Walters, Benjamin; Baker, Amelia; Guda, Teja; Dion, Gregory R
- Abstract
Objective: To investigate changes in neuroregenerative pathways with vocal fold denervation in response to vocal fold augmentation. Methods: Eighteen Yorkshire crossbreed swine underwent left recurrent laryngeal nerve transection, followed by observation or augmentation with carboxymethylcellulose or calcium hydroxyapatite at two weeks. Polymerase chain reaction expression of genes regulating muscle growth (MyoD1, MyoG and FoxO1) and atrophy (FBXO32) were analysed at 4 and 12 weeks post-injection. Thyroarytenoid neuromuscular junction density was quantified using immunohistochemistry. Results: Denervated vocal folds demonstrated reduced expression of MyoD1, MyoG, FoxO1 and FBXO32, but overexpression after augmentation. Healthy vocal folds showed increased early and late MyoD1, MyoG, FoxO1 and FBXO32 expression in all animals. Neuromuscular junction density had a slower decline in augmented compared to untreated denervated vocal folds, and was significantly reduced in healthy vocal folds contralateral to augmentation. Conclusion: Injection augmentation may slow neuromuscular degeneration pathways in denervated vocal folds and reduce compensatory remodelling in contralateral healthy vocal folds.
- Subjects
RECURRENT laryngeal nerve; HYDROXYAPATITE; STRUCTURAL models; ANIMAL experimentation; IMMUNOHISTOCHEMISTRY; NEUROMUSCULAR diseases; VOCAL cords; SWINE; GENE expression; ATROPHY; COMPARATIVE studies; DRUG synergism; BONE remodeling; RESEARCH funding; POLYMERASE chain reaction; CELLULOSE
- Publication
Journal of Laryngology & Otology, 2024, Vol 138, Issue 2, p196
- ISSN
0022-2151
- Publication type
Article
- DOI
10.1017/S0022215123001135