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- Title
Espermatozoides caprinos criopreservados em meio à base de leite desnatado acrescido de glutationa reduzida.
- Authors
Trindade Soares, Adriana; Valcácia Silva, Sildivane; Costa Almeida, Felipe; de Araújo Lemos, Paula Fernanda Barbosa; Ferreira Nunes, José; Alves Peixoto, Christina; Madalena Pessoa Guerra, Maria
- Abstract
Aiming to evaluate in vitro effect of different concentrations of glutathione reduced (GSH) in skimmedmilk and glycerol 7% it was used semen from five Boer bucks. After collect and evaluation, a pool of samples was diluted in skimmed-milk and glycerol 7% plus antioxidant: G1) Control; G2) GSH 2mM mL-1; G3) GSH 5mM mL-1 and G4) GSH 7mM mL-1. Samples were frozen in straws (0.25mL) and stored at - 196oC. After thawing, samples were subjected to integrity of the plasma membrane (iMP) and acrosomal (iAc), mitochondrial membrane potential (MMP), kinematic and ultrastructure analysis. Control and GSH (2, 5 and 7mM mL-1) groups did no differ (P>0.05) in iMP, iAc, PMM and kinematic parameters. In the ultrastructural analysis, percentages of acrosome and plasma membrane (tail and head region) intact did not differ (P>0.05) between groups. However, Control group had higher percentage (P<0.05) of gametes with intact axonemes than those of GSH (2, 5 and 7mM mL-1) groups. Higher percentage (P<0.05) of sperms with intact mitochondrias were observed on Control group than those of GSH (5 and 7mM mL-1). It can be concluded that the GSH (2, 5 and 7mM mL-1) addition in skimmed-milk diluent to freeze goat semen did not preserve sperm integrity.
- Subjects
PHYSIOLOGICAL effects of glutathione; SEMEN; GOATS; PHYSIOLOGY; KINEMATICS; CELL membranes; CONTROL groups
- Publication
Ciência Rural, 2011, Vol 41, Issue 11, p1991
- ISSN
0103-8478
- Publication type
Article
- DOI
10.1590/S0103-84782011001100023