We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Macrophages largely contribute to heterologous anti- Propionibacterium acnes antibody-mediated protection from Actinobacillus pleuropneumoniae infection in mice.
- Authors
Ma, Qiuyue; Sun, Changjiang; Yang, Feng; Wang, Lei; Qin, Wanhai; Xia, Xiaojing; Feng, Xin; Du, Chongtao; Gu, Jingmin; Han, Wenyu; Lei, Liancheng
- Abstract
Actinobacillus pleuropneumoniae is the causative agent of acute and chronic pleuropneumonia. Propionibacterium acnes is a facultative anaerobic gram-positive corynebacterium. We have previously found that anti- P. acnes antibodies can prevent A. pleuropneumoniae infections in mice. To investigate the role of macrophages in this process, affinity-purified anti- P. acnes IgG and anti- A. pleuropneumoniae IgG were used in opsonophagocytosis assays. Additionally, the efficacy of passive immunization with P. acnes serum against A. pleuropneumoniae was tested in macrophage-depleted mice. It was found that anti- P. acnes IgG had an effect similar to that of anti- A. pleuropneumoniae IgG ( P > 0.05), which significantly promotes phagocytosis of A. pleuropneumoniae by macrophages ( P < 0.01). It was also demonstrated that, after passive immunization with anti- P. acnes serum, macrophage-replete mice had the highest survival rate (90%), whereas the survival rate of macrophage-depleted mice was only 40% ( P < 0.05). However, macrophage-depleted mice that had been passively immunized with naïve serum had the lowest survival rate (20%), this rate being lower than that of macrophage-replete mice that had been passively immunized with naïve serum. Overall, anti- P. acnes antibodies did not prevent A. pleuropneumoniae infection under conditions of macrophage depletion ( P > 0.05). Furthermore, in mice that had been passively immunized with anti- P. acnes serum, macrophage depletion resulted in a greater A. pleuropneumoniae burden and more severe pathological features of pneumonia in lung tissues than occurred in macrophage-replete mice. It was concluded that macrophages are essential for the process by which anti- P. acnes antibody prevents A. pleuropneumoniae infection in mice.
- Subjects
MACROPHAGES; CUTIBACTERIUM acnes; ACTINOBACILLUS pleuropneumoniae; IMMUNOGLOBULINS; LABORATORY mice; PLEUROPNEUMONIA; GRAM-positive bacteria; THERAPEUTICS
- Publication
Microbiology & Immunology, 2015, Vol 59, Issue 3, p166
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/1348-0421.12240