We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Plk1 inhibition leads to a failure of mitotic division during the first mitotic division in pig embryos.
- Authors
Zhang, Zixiao; Chen, Changchao; Cui, Panpan; Liao, Yaya; Yao, Lingyun; Zhang, Yue; Rui, Rong; Ju, Shiqiang
- Abstract
Purpose: This study was conducted to examine the dynamic distribution of polo-like 1 kinase (Plk1) and the possible role it plays in first mitotic division during early porcine embryo development. Methods: Indirect immunofluorescence and confocal microscopy imaging techniques combined with western blot analyses were used to study the dynamic expression and subcellular localization of Plk1 protein in pig parthenogenetic embryos. Finally, a selective Plk1 inhibitor, GSK461364, was used to evaluate the potential role of Plk1 during this special stage. Results: The results showed that Plk1 upon expression exhibited specific dynamic intracellular localization, which closely correlated with the α-tubulin distribution during the first mitotic division. GSK461364 treatment resulted in cleavage failure, with majority of the GSK461364-treated embryos being arrested in prometaphase. Further results of the subcellular structure examination showed that GSK461364 treatment led to a significantly higher proportion of the treated embryos having abnormal spindles and misarranged chromosomes at the prometaphase stage. Conclusions: Thus, these results indicated that Plk1 is essential for porcine embryos to complete the first mitotic division. Furthermore, Plk1 regulation was associated with effects on spindle assembly and chromosome arrangement.
- Subjects
SWINE embryology; POLO-like kinases; CELL division; PARTHENOGENESIS in animals; ENZYME inhibitors
- Publication
Journal of Assisted Reproduction & Genetics, 2017, Vol 34, Issue 3, p399
- ISSN
1058-0468
- Publication type
Article
- DOI
10.1007/s10815-016-0864-4