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- Title
Cloning and Homologous Expression of Novel Lignin Peroxidase Genes in the White-Rot Fungus Phanerochaete sordida YK-624.
- Authors
Sugiura, Tatsuki; Yamagishi, Kenji; Kimura, Toshiyuki; Nishida, Tomoaki; Kawagishi, Hirokazu; Hirai, Hirofumi
- Abstract
The article presents a study on the cloning and homologous expression of YK-LiPs genes in Phanerochaete sordida YK-624. It notes that lignin is a phenylpropanoid polymer which constitutes 20-30% of woody plant cell walls and can be degraded by multiple extracellular enzymes. With this, various methods are used such as strains of P. sordida, construction of ylpA expression plasmid, and cultivation in recombinant LiP suppression medium. The result shows successful homologous expression of YK-LiP2.
- Subjects
PHANEROCHAETE; CLONING; GENE expression; LIGNINS; ANTISENSE DNA; POLYMER research; EXTRACELLULAR enzymes; PLASMIDS
- Publication
Bioscience, Biotechnology & Biochemistry, 2009, Vol 73, Issue 8, p1793
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1271/bbb.90152