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- Title
Protective effects of genistein in homocysteine-induced endothelial cell inflammatory injury.
- Authors
Han, Shengbo; Wu, Hui; Li, Wenxue; Gao, Pan
- Abstract
Hyperhomocysteinemia is a risk factor for cardiovascular disease and the mechanism of homocysteine (HCY)-induced vascular endothelial cell injury has been intensively studied for many years. Recently, a large number of studies have shown inhibitory effects of genistein (GEN), a soy isoflavone, in the process of endothelial cell injury. In the present study, the protective effects of GEN in HCY-induced endothelial cell inflammatory injury were investigated. A model of HCY-induced endothelial cell (ECV-304) inflammatory injury was established in vitro, and the protective effect of GEN in this procession was explored. According to our results, GEN protected HCY-induced endothelial cell from viability decreases, meanwhile prevented the changes of cell morphology and the production of reactive oxygen species (ROS). The expression of NF- kB P-65, IL-6, and ICAM-1 was all down-regulated. During the HCY-induced endothelial cell injury, the endothelial cell apoptosis and proliferation disorder were alleviated. Therefore, we conclude that HCY-induced endothelial cell inflammatory injury could be blocked by GEN. The present findings suggest that GEN protects HCY-induced endothelial cell inflammatory injury may through reducing the release of ROS, inhibiting NF- kB activation, down-regulating the expression of cytokine IL-6 and adhesion molecules ICAM-1, avoiding inflammatory cells and platelet adhesion, accordingly, leading to a balance of endothelial cell proliferation and apoptosis.
- Subjects
GENISTEIN; HOMOCYSTEINE; VASCULAR endothelial cells; HYPERHOMOCYSTEINEMIA; CARDIOVASCULAR diseases risk factors
- Publication
Molecular & Cellular Biochemistry, 2015, Vol 403, Issue 1/2, p43
- ISSN
0300-8177
- Publication type
Article
- DOI
10.1007/s11010-015-2335-0