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- Title
Heterogeneity and evolution of tumour immune microenvironment in metastatic gastroesophageal adenocarcinoma.
- Authors
Wang, Wei; Ye, Liu-Fang; Bao, Hua; Hu, Ming-Tao; Han, Ming; Tang, Hai-Meng; Ren, Chao; Wu, Xue; Shao, Yang; Wang, Feng-Hua; Zhou, Zhi-Wei; Li, Yu-Hong; Xu, Rui-Hua; Wang, De-Shen
- Abstract
Background: Tumour immune microenvironment heterogeneity is prevalent in numerous cancers and can negatively impact immunotherapy response. Immune heterogeneity and evolution in gastroesophageal adenocarcinoma (GEA) have not been studied in the past. Methods: Together with a multi-region sampling of normal, primary and metastatic tissues, we performed whole exome sequencing, TCR sequencing as well as immune cell infiltration estimation through deconvolution of gene expression signals. Results: We discovered high TCR repertoire and immune cell infiltration heterogeneity among metastatic sites, while they were homogeneous among primary and normal samples. Metastatic sites shared high levels of abundant TCR clonotypes with blood, indicating immune surveillance via blood. Metastatic sites also had low levels of tumour-eliminating immune cells and were undergoing heavy immunomodulation compared to normal and primary tumour tissues. There was co-evolution of neo-antigen and TCR repertoire, but only in patients with late diverging mutational evolution. Co-evolution of TCR repertoire and immune cell infiltration was seen in all except one patient. Conclusions: Our findings revealed immune heterogeneity and co-evolution in GEA, which may inform immunotherapy decision-making.
- Subjects
TUMOR microenvironment; HETEROGENEITY; METASTASIS; ADENOCARCINOMA; GENE expression
- Publication
Gastric Cancer, 2022, Vol 25, Issue 6, p1017
- ISSN
1436-3291
- Publication type
Article
- DOI
10.1007/s10120-022-01324-7