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- Title
Nematode-specific cadherin CDH-8 acts as a receptor for Cry5B toxin in <italic>Caenorhabditis elegans</italic>.
- Authors
Peng, Donghai; Wan, Danfeng; Cheng, Chunsheng; Ye, Xiaobo; Sun, Ming
- Abstract
Parasitic nematodes of animals and plants cause worldwide devastating impacts on people’s lives and agricultural crops. The crystal protein Cry5B produced by <italic>Bacillus thuringiensis</italic> has efficient and specific activity against a wide range of nematodes. However, the action mode of this toxin has not yet been thoroughly determined. Here, a nematode-specific cadherin CDH-8 was demonstrated to be a receptor for Cry5B toxin by using <italic>Caenorhabditis elegans</italic> as a model, providing evidence that the cadherin mutant worm <italic>cdh-8(RB815)</italic> possesses significant resistance to Cry5B, and the CDH-8 fragments bind specifically to Cry5B. Furthermore, CDH-8 was identified to be required for the oligomerization of Cry5B toxin in vivo and contribute to the internalization and pore formation of Cry5B in nematode cells. This study will facilitate a better understanding of the action mode of nematicidal Cry toxins and help the design of Cry toxin-based products for the control of plant or animal parasitic nematodes.
- Subjects
NEMATODES; CAENORHABDITIS elegans; CROPS; CADHERINS; PARASITES
- Publication
Applied Microbiology & Biotechnology, 2018, Vol 102, Issue 8, p3663
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-018-8868-x