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- Title
Production of a novel compound, 10,12-dihydroxystearic acid from ricinoleic acid by an oleate hydratase from Lysinibacillus fusiformis.
- Authors
Seo, Min-Ho; Kim, Kyoung-Rok; Oh, Deok-Kun
- Abstract
A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction conditions for the production of 10,12-dihydroxystearic acid were optimized as follows: pH 6.5, 30 °C, 15 g l ricinoleic acid, 9 mg ml of enzyme, and 4 % ( v/ v) methanol. Under the optimized conditions, the enzyme produced 13.5 g l 10,12-dihydroxystearic acid without detectable byproducts in 3 h, with a conversion of substrate to product of 90 % ( w/ w) and a productivity of 4.5 g l h. The emulsifying activity of 10,12-dihydroxystearic acid was higher than that of oleic acid, ricinoleic acid, stearic acid, and 10-hydroxystearic acid, indicating that 10,12-dihydroxystearic acid can be used as a biosurfactant.
- Subjects
OLEIC acid; CHEMICAL structure; MASS spectrometry; GAS chromatography; NUCLEAR magnetic resonance; STEARIC acid; BIOSURFACTANTS
- Publication
Applied Microbiology & Biotechnology, 2013, Vol 97, Issue 20, p8987
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-013-4728-x