We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
EP342 BIOCHEMICAL PROFILING OF PORCINE BURN WOUND HEALING FOLLOWING TREATMENT WITH ACELLULAR FISH SKIN GRAFTS.
- Authors
Rolfsson, Ottar; Kotronoulas, Aristotelis; Christiansen, Christian; de Lomana, Adrian Lopez Garcia; Stamatakis, Giorgios; Heijink, Marieke; Giera, Martin; Samiotaki, Martina; Kjartansson, Hilmar; Stone II, Randolph
- Abstract
Aim: Acellular fish skin graft (AFSG) and fetal bovine skin graft (FBS) are commonly used biomaterials with molecular and structural properties that support wound healing resulting in less scar tissue formation and pain. However, how AFSG modulates the biochemical profiles of wound healing is not understood. We aim to understand how AFSG influences lipid, metabolite, and protein profiles of burn wound healing using a mass spectrometry-based data-driven approach. Method: Partial (PTBW) and full (FTBW) thickness burn wounds were created on Yorkishire pigs. PTBW were treated with AFSG or FBS; FTBW were treated with AFSG or cadaver skin, followed by a split-thickness skin graft. Punch biopsies were collected over time to measure approximately 50 derivatives of EPA, DHA, arachidonic acid, linoleic acid metabolic pathways by targeted UPLC-MS/MS. Untargeted metabolomics using UPLC/Q-TOF-MS, and label-free quantitative shotgun proteomics by UPLC/Orbitrap-MS were also performed. Results / Discussion: In the PTBW, EPA and DHA derivatives, including 18-HEPE and 17-HDHA, were significantly increased at day 7 in the AFSG-treated wounds. A similar trend was observed in FTBW. Prostaglandin F2a and its 15-keto derivative from the AA pathway and 13-HODE and 13-HOTrE from the LA pathway increased significantly at day 7, independent of treatment. Untargeted metabolomics revealed differences in amino acid, nucleoside, and carbohydrate profiles only at day 7, irrespective of wound type. Changes in proteomic profiles were primarily characterized by downregulation of proteins involved in keratinization and the immune response in both PTBW and FTBW. Conclusion: Burn wound treatment with AFSG leads to earlier formation of lipid mediators involved in the resolution of inflammation partly driven by changes to protein and/or cellular composition of the wound.
- Subjects
ITALY; LIPID metabolism; PROTEIN metabolism; BIOMARKERS; WOUND healing; SKIN grafting; XENOGRAFTS; BURNS &; scalds; METABOLOMICS; CONFERENCES &; conventions; FISHES; MASS spectrometry; WOUND care
- Publication
Journal of Wound Management, 2023, Vol 24, Issue 2, p223
- ISSN
2788-5771
- Publication type
Article