We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Burkholderia phymatum improves salt tolerance of symbiotic nitrogen fixation in Phaseolus vulgaris.
- Authors
Talbi, Chouhra; Argandoña, Montserrat; Salvador, Manuel; Alché, Juan; Vargas, Carmen; Bedmar, Eulogio; Delgado, María
- Abstract
Background and aims: Burkholderia phymatum strain GR01 is a recently reported common bean ( Phaseolus vulgaris) symbiont isolated from nodules of plants grown in semi-arid soils in Morocco. The osmotolerance of B. phymatum GR01N under free-living and in symbiotic association with P. vulgaris was investigated in this study. Methods: The osmotolerance of B. phymatum GR01N was checked by growing cells in the presence of varying concentrations of NaCl or sucrose, and the cellular solutes were analyzed in cell extracts by C-nuclear magnetic resonance (NMR) spectroscopy. Nodule occupancy was checked in P. vulgaris grown in the presence of 25, 35 or 50 mM NaCl and inoculated with a mixture of B. phymatum GR01N and R. tropici CIAT899R cells. The effect of salt stress on nodule biomass, plant dry weight, plant nitrogen content and leghaemoglobin content of nodules was also analyzed in plants inoculated with either B. phymatum GR01N or R. tropici CIAT899R and grown in the presence of 25 or 35 mM NaCl. Results: Burkholderia phymatum strain GR01N showed increased tolerance to osmotic stress under free-living conditions as compared to the reference strain R. tropici CIAT899R. Strain GR01N accumulated trehalose, mannitol and alanine in response to saline stress, suggesting their role in the observed osmoloterance. Under conditions of saline stress, P. vulgaris plants nodulated by B. phymatum GR01N showed increased plant dry weight and nitrogen fixation, when compared to those inoculated with R. tropici CIAT899R. Nodule competition assays revealed that B. phymatum GR01N had higher levels of nodule occupancy than R. tropici CIAT899R in P. vulgaris plants grown under saline conditions. Conclusions: Burkholderia phymatum strain GR01N displays a remarkable osmotolerance under free-living and symbiotic conditions.
- Subjects
KIDNEY bean; MICROBIOLOGY; BURKHOLDERIA; EFFECT of salts on plants; BIOCOMPATIBILITY; NITROGEN fixation; SYMBIOSIS
- Publication
Plant & Soil, 2013, Vol 367, Issue 1/2, p673
- ISSN
0032-079X
- Publication type
Article
- DOI
10.1007/s11104-012-1499-6