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- Title
Isolation and cDNA cloning of an Em‐like protein from mung bean (Vigna radiata) axes.
- Authors
Manickam, Ayyanar; van Damme, Els J. M.; Kalaiselvi, Kandasamy; Verhaert, Peter; Peumans, Willy J.
- Abstract
Until now no ‘early‐methionine‐labelled’ (Em) proteins have been reported in the Fabaceae. To check whether a previously isolated low‐molecular mass albumin from dry mung bean embryonic axes possibly corresponded to an Em‐like protein, the protein was purified, sequenced and its cDNA clone isolated and characterized. N‐terminal sequencing of cyanogen bromide cleavage products of the protein revealed homology with previously described Em‐like proteins from other species. Analysis of cDNA clones encoding the mung bean Em protein revealed the presence of two classes of Em proteins and confirmed their homology to the previously characterized Em‐like proteins. In vivo labelling and northern blot analysis further demonstrated that the mung bean protein is synthesized during early germination of the axes and that abscisic acid (ABA) extends its synthesis. It appears, therefore, that legumes also contain maturation‐specific, ABA‐responsive Em‐like proteins.
- Subjects
MUNG bean; MOLECULAR cloning; LEGUMES; PROTEINS; ABSCISIC acid; GERMINATION
- Publication
Physiologia Plantarum, 1996, Vol 97, Issue 3, p524
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1996.tb00512.x