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- Title
MiR-129 reduces CDDP resistance in gastric cancer cells by inhibiting MAPK3.
- Authors
CAO, H. Y.; XIAO, C. H.; LU, H. J.; YU, H. Z.; HONG, H.; GUO, C. Y.; YUAN, J. F.
- Abstract
OBJECTIVE: Abnormal expression of mitogen-activated protein kinase 3 (MAPK3) is related to invasion, metastasis, and drug resistance of multiple tumor cells. MiR-129 expression is associated with gastric cancer. Bioinformatics analysis showed a targeting relation between miR-129 and MAPK3. This study investigated whether miR-129 plays a role in regulating MAPK3 expression, affecting proliferation, apoptosis, and cisplatin (CDDP) resistance of gastric cancer cells. MATERIALS AND METHODS: The dual-luciferase reporter gene assay was used to assess the targeted regulation between miR-129 and MAPK3. The expression of miR-129 and MAPK3 in CDDP-resistant cell line MGC-803/ CDDP and the parental MGC-803 cells was measured. MGC-803/CDDP cells were cultured in vitro and divided into miR-NC group and miR-129 mimic group. The expression of MAPK3 and p-MAPK3 protein were detected by Western blot and the effect of CDDP treatment on cell apoptosis and proliferation was detected by flow cytometry. RESULTS: There was a targeted regulation relation between miR-129 and MAPK3 mRNA. MiR-129 expression in MGC-803/CDDP cells was significantly lower than that in MGC-803 cells and the expression of MAPK3 mRNA and protein was significantly higher than that in MGC- 803 cells. Compared with miR-NC group, the expression of MAPK3 and p-MAPK3 in MHC-803/ CDDP cells in miR-129 mimic transfection group was significantly decreased, with increased cell apoptosis and reduced cell proliferation. CONCLUSIONS: The decreased expression of miR-129 and the up-regulation of MAPK3 are associated with CDDP resistance in gastric cancer cells. Overexpression of miR-129 inhibits MAPK3 expression and cell proliferation, it induces cell apoptosis and reduces CDDP resistance.
- Subjects
DRUG resistance in cancer cells; CANCER cells; STOMACH cancer; CANCER stem cells; MITOGEN-activated protein kinases; APOPTOSIS
- Publication
European Review for Medical & Pharmacological Sciences, 2019, Vol 23, Issue 15, p6478
- ISSN
1128-3602
- Publication type
Article