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- Title
High-throughput analysis of high-molecular weight glutenin subunits in 665 wheat genotypes using an optimized MALDI-TOF–MS method.
- Authors
Jang, You-Ran; Kim, Sewon; Sim, Jae-Ryeong; Lee, Su-Bin; Lim, Sun-Hyung; Kang, Chon-Sik; Choi, Changhyun; Goo, Tae-Won; Lee, Jong-Yeol
- Abstract
Gluten protein composition determines the rheological characteristics of wheat dough and is influenced by variable alleles with distinct effects on processing properties. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF–MS), we determined the high-molecular weight glutenin subunit (HMW-GS) composition of 665 wheat genotypes employed in breeding programs in South Korea. We identified 22 HMW-GS alleles, including 3 corresponding to the Glu-A1 locus, 14 to Glu-B1, and 5 to Glu-D1. The Glu-1 quality score, which is an important criterion for high-quality wheat development, was found to be 10 for 105/665 (15.79%) of the studied genotypes, and included the following combinations of HMW-GS: 2*, 7 + 8, 5 + 10; 2*, 17 + 18, 5 + 10; 1, 7 + 8, 5 + 10; and 1, 17 + 18, 5 + 10. To select wheat lines with the 1Bx7 overexpression (1Bx7OE) subunit, which is known to have a positive effect on wheat quality, we used a combination of MALDI-TOF–MS and published genotyping markers and identified 6 lines carrying 1Bx7OE out of the 217 showing a molecular weight of 83,400 Da, consistent with 1Bx7G2 and 1Bx7OE. This study demonstrates that the MALDI-TOF–MS method is fast, accurate, reliable, and effective in analyzing large numbers of wheat germplasms or breeding lines in a high-throughput manner.
- Subjects
SOUTH Korea; GLUTELINS; TIME-of-flight mass spectrometry; WHEAT; WHEAT breeding; FLOUR; GENOTYPES
- Publication
3 Biotech, 2021, Vol 11, Issue 2, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-020-02637-z