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- Title
Infraspecific identity of the wild Brassica nigra (L.) Koch. using morphological, cytogenetics and molecular (nuclear and chloroplast) approaches.
- Authors
Amer, Wafaa M.; Shoulkamy, Mahmoud A.; Abd El-Baset, Hadeer D.; Faried, Ahmed M.
- Abstract
Egyptian flora is still hosting the wild diploid Brassica nigra (L.) Koch. (Brassicaceae); as one of the most important wild crop relatives of the cultivated Brassica crops. Despite the field and herbarium observations indicating that the populations of this species showed considerable morphological diversity, the taxonomic identity of these variations is not yet resolved. This study covered the morphology, the cytogenetics and the genome diversity at the intraspecies level to resolve the taxonomic identity and their genetic relationships. Nine representative populations were morphologically investigated using 70 macro-and micro-morphological characters. The morphological results confirmed the presence of two varieties (var. bracteolata and var. nigra), and three forms, while the chromosome investigation revealed the presence of 2n=16), with notable karyotyping diversity among the studied varieties where satellite was observed on the chromosome pair no. "6" distinguished var. bracteolata from var. nigra. The retrieved accession numbers of the chloroplast (rps, ropC & trnK) and nuclear (ITS) markers have been deposited in the EMBL/GenBank/DDBJ nucleotide sequence data libraries; then compared with that of B. nigra from Gene-Bank database search via Basic Local Alignment Search Tool (BLAST). The constructed cladogram based on molecular markers supported the morphological and cytological results and confirmed the presence of the two varieties (bracteolata and nigra). This study recommends combining different taxonomic approaches in resolving the taxonomic identity of morphologically different wild B. nigra populations.
- Subjects
MUSTARD; CYTOGENETICS; DATA libraries; NUCLEOTIDE sequence; BIOLOGICAL specimens; DATABASE searching; BOTANICAL specimens; KARYOTYPES
- Publication
Jordan Journal of Biological Sciences, 2022, Vol 15, Issue 3, p489
- ISSN
1995-6673
- Publication type
Article
- DOI
10.54319/jjbs/150319