We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Prokaryotic Expression of Murine Cellular Prion Protein for In vitro Evaluation.
- Authors
Hoang-Anh Le-Dao; Yen-Vy Le-Nguyen; Hieu Tran-Van
- Abstract
M cell (Microfold cell) targeting is a potential strategy for oral vaccine development due to its role in presenting antigen to mucosal lymphoid tissue. Numerous researches have focused on directing vaccine antigens to M cell receptors, based on their affinity with specific ligands. This study was aimed to biosynthesize murine cellular prion protein (mPrPC), a highly expressed receptor on the M cell apical surface. The mPrPC coding sequence was obtained by PCR for construction of pGEX-5X-1-mprpc plasmid. Then, recombinant plasmid was induced for expression in E. coli BL21(DE3) cells by IPTG. The overexpression of mPrPC was confirmed by SDS-PAGE and Western blotting. Since the expressed protein was found to remain mostly in insoluble fraction, DTT and glycerol were used to improve the solubility. This soluble, recombinant mPrPC could be used for in vitro evaluation, and also on further research for developing antigen targeting M cell.
- Subjects
CELL receptors; ESCHERICHIA coli; MUCOUS membranes; LYMPHOID tissue; PROTEINS; PRIONS
- Publication
Jordan Journal of Biological Sciences, 2022, Vol 15, Issue 3, p361
- ISSN
1995-6673
- Publication type
Article
- DOI
10.54319/jjbs/150303