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- Title
Expresión del citocromo p450 1A1 en enfermedad tumoral de tiroides.
- Authors
Gallegos-Vargas, José Antonio; López-Chavira, Adelaido; Floriano-Sánchez, Esaú; Rivera-Martínez, César Gamaliel; Ronquillo-Sánchez, Dolores; Prestegui-Martel, Berenice; Sánchez-Roldán, Jaime; Mellnyk, Allá
- Abstract
Background: Thyroid tumor pathology is common, with a worldwide prevalence of nodules of 5% in women and 1% in males, 5 to 15% are malignant; is the most common cause of neck surgery at the Central Military Hospital. Cytochrome P450 (CYP) superfamily of enzymes is priimportant in the metabolism of drugs and toxic, presumably active carcinogens. The CYP 1A1 induced by aromatic hydrocarbons has been associated with cancer; studies in thyroid nodules, benign tumor pathology and cancer have not had any definite conclusions. Objective: To verify if the expression of CYP1A1 gene is over-regulated in malignant thyroid disease regarding benign. Material and method: An observational, cross-sectional, descriptive and analytical study in which 64 tissue samples were collected, 32 of malignant disease, 32 of benign disease; RNA (Trizol) was extracted and its integrity was verified; RT-PCR was performed for gene expression of CYP 1A1, statistical was applied. Results: RNA was extracted from 64 samples, RT-PCR was performed, with n=59 final; in statistical analysis p=0.3048 was obtained, although not statistically significant, an over-expression of CYP 1A1 relative in malignant versus benign (factor=1.968) was confirmed, confirming our hypothesis. Conclusions: We hypothesize an over-expression of CYP 1A1 in thyroid pathology, this was verified, gene expression is increased relative to the endogenous gene GAPDH, both malignancy and benign; even without a significant difference between these, there is a numeric over-expression on CYP 1A1 in malignancy. Much more research is needed in genomic tumor disease of head and neck.
- Publication
Anales de Otorrinolaringología Mexicana, 2015, Vol 60, Issue 3, p140
- ISSN
1665-5672
- Publication type
Article