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- Title
Investigating the in vitro antibacterial efficacy of composite bone cement incorporating natural product-based monomers and gentamicin.
- Authors
Kan, Yu-Chen; Guo, Rui; Xu, Yang; Han, Lu-Yang; Bu, Wen-Han; Han, Long-Xu; Chu, Jian-Jun
- Abstract
Objective: The objective of this study is to investigate the impact of four natural product extracts, namely, aloe-emodin, quercetin, curcumin, and tannic acid, on the in vitro bacteriostatic properties and biocompatibility of gentamicin-loaded bone cement and to establish an experimental groundwork supporting the clinical utility of antibiotic-loaded bone cements (ALBC). Methods: Based on the components, the bone cement samples were categorized as follows: the gentamicin combined with aloe-emodin group, the gentamicin combined with quercetin group, the gentamicin combined with curcumin group, the gentamicin combined with tannic acid group, the gentamicin group, the aloe-emodin group, the quercetin group, the curcumin group, and the tannic acid group. Using the disk diffusion test, we investigated the antibacterial properties of the bone cement material against Staphylococcus aureus (n = 4). We tested cell toxicity and proliferation using the cell counting kit-8 (CCK-8) and examined the biocompatibility of bone cement materials. Results: The combination of gentamicin with the four natural product extracts resulted in significantly larger diameters of inhibition zones compared to gentamicin alone, and the difference was statistically significant (P < 0.05). Except for the groups containing tannic acid, cells in all other groups showed good proliferation across varying time intervals without displaying significant cytotoxicity (P < 0.05). Conclusion: In this study, aloe-emodin, quercetin, curcumin, and tannic acid were capable of enhancing the in vitro antibacterial performance of gentamicin-loaded bone cement against S. aureus. While the groups containing tannic acid displayed moderate cytotoxicity in in vitro cell culture, all other groups showed no discernible cytotoxic effects.
- Subjects
ANTIBIOTICS; COMBINATION drug therapy; IN vitro studies; TANNINS; MICROBIAL sensitivity tests; RESEARCH funding; QUINONE; CELL proliferation; BIOLOGICAL products; STAPHYLOCOCCUS aureus; METHICILLIN-resistant staphylococcus aureus; DESCRIPTIVE statistics; QUERCETIN; BIOMEDICAL materials; CELL culture; DRUG efficacy; GENTAMICIN; BONE cements; CURCUMIN; COMPARATIVE studies; PHARMACODYNAMICS
- Publication
Journal of Orthopaedic Surgery & Research, 2024, Vol 19, Issue 1, p1
- ISSN
1749-799X
- Publication type
Article
- DOI
10.1186/s13018-024-04646-7