We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The effect of subminimal inhibitory concentrations of antibiotics on virulence factors expressed by Staphylococcus aureus biofilms.
- Authors
Haddadin, R. N. S.; Saleh, S.; Al-Adham, I. S. I.; Buultjens, T. E. J.; Collier, P. J.
- Abstract
Aims: The effect of subminimal inhibitory concentrations (sub-MICs) of cefalexin, ciprofloxacin and roxithromycin was investigated on some virulence factors [e.g. coagulase, Toxic Shock Syndrome Toxin 1 (TSST-1) and biofilm formation] expressed by Staphylococcus aureus biofilms. Methods and Results: Biofilms were grown with and without the presence of 1/16 MIC of antibiotics on Sorbarod filters. Eluate supernatants were collected, and coagulase and TSST-1 production were evaluated. Coagulase production was reduced in eluates exposed to roxithromycin when compared to control, while TSST-1 production was reduced in biofilms exposed to cefalexin and to a lesser extent, ciprofloxacin. In addition, the ability of Staph. aureus to produce biofilm in microtitre plates in the presence of sub-MIC antibiotics indicated that cefalexin induced biofilm formation at a wide range of sub-MICs. TSST-1 produced from the challenged and control biofilms was purified, and its proliferative activity was studied on single cell suspension of mouse splenocytes using MTS/PMS assay. No significant difference in the activity between the treated toxin and the control has been observed. Conclusions: Antibiotics at sub-MIC levels interfere with bacterial biofilm virulence expression depending on the type and concentration of antibiotic used. Significance and Impact of the Study: The establishment of sub-MICs of antibiotics in clinical situations may result in altered virulence states in pathogenic bacteria.
- Subjects
STAPHYLOCOCCUS aureus; ANTIBIOTICS; BIOFILMS; MICROBIAL aggregation; CIPROFLOXACIN; TOXIC shock syndrome toxin-1; CELL suspensions; COAGULASE; LABORATORY mice
- Publication
Journal of Applied Microbiology, 2010, Vol 108, Issue 4, p1281
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1111/j.1365-2672.2009.04529.x