We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Protective Effects of Total Saponins of Panax Notoginseng on Steroid-Induced Avascular Necrosis of the Femoral Head In Vivo and In Vitro.
- Authors
Yuan, Heng-feng; Pan, Jian-feng; Li, Shuo; Guo, Chang-an; Liu, Shu-hao; Yan, Zuo-qin
- Abstract
This research was designed to investigate the protective effects of TSPN on steroid-induced avascular necrosis of the femoral head (ANFH) and the likely mechanisms of those effects. As an in vivo study, TSPN was shown to be protective against steroid-induced ANFH due to the upregulation of VEGF-A. Furthermore, TSPN attenuated the apoptosis of osteocytes and reduced the expression of Caspase-3 relative to the model group. As an in vitro study, TSPN exerted a concentration-dependent protective effect against apoptosis in MC3T3-E1 cells. Moreover, TSPN (at a dose of 100 μg/mL) significantly reversed the dexamethasone-induced augmentation of Caspase-3 expression and activity. Therefore, our study demonstrated that TSPN had a protective effect against steroid-induced ANFH that was related to the upregulation of VEGF-A and the inhibition of apoptosis and Caspase-3 activation.
- Subjects
OSTEONECROSIS; ANALYSIS of variance; ANIMAL experimentation; APOPTOSIS; CELL physiology; GINSENG; RATS; RESEARCH funding; SOAP; STEROIDS; WESTERN immunoblotting; PROTEASE inhibitors; VASCULAR endothelial growth factors; FEMUR head; DATA analysis software; DESCRIPTIVE statistics; IN vitro studies; IN vivo studies; DIAGNOSIS
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2015, Vol 2015, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2015/165679