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- Title
Preparation of Homogeneous Solid Samples for Reproducible and Quantitative MALDI.
- Authors
Ahn, Sung Hee; Park, Kyung Man; Moon, Jeong Hee; Lee, Seong Hoon; Kim, Myung Soo
- Abstract
Preparing samples with good homogeneity is one of the requirements for generating reproducible matrix-assisted laser desorption ionization ( MALDI) spectra and for using them in analyte quantification. Thus far, we have not been successful in producing homogeneous solid samples from popular matrixes apart from α-cyano-4-hydroxycinnamic acid ( CHCA). In this work, we demonstrate the production of an outwardly homogeneous solid sample by loading a methanol solution of a matrix into a shallow reservoir on a coated MALDI sample plate and then vacuum-drying it. Out of ten popular matrixes tested, seven yielded homogeneous samples. These were 9-aminoacridine, 6-aza-2-thiothymine, CHCA, 2,5-dihydroxybenzoic acid, ferulic acid, sinapinic acid, and 2,4,6-trihydroxyacetophenone. For MALDI with these matrixes, linear calibration curves covering wide dynamic ranges were acquired. Performances of these matrixes in various aspects of analyte quantification have been investigated.
- Subjects
DESORPTION ionization mass spectrometry; METHANOL; CHEMICAL synthesis; HYDROXYCINNAMIC acids; FERULIC acid; HOMOGENEITY
- Publication
Bulletin of the Korean Chemical Society, 2016, Vol 37, Issue 4, p458
- ISSN
0253-2964
- Publication type
Article
- DOI
10.1002/bkcs.10704