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- Title
INFLUENCE OF COLLECTION TIME ON THE DETERMINATION OF ROOT EXUDATES IN FRAXINUS MANDSHURICA BY THE METABOLOMICS METHOD.
- Authors
LI, L. Y.; FENG, C. X.; ZHANG, Y. D.
- Abstract
Root exudates play an important role in the control of soil ecology because they are major sources of organic carbon and energy for plants, and they drive the carbon cycle of forest ecosystems. However, qualitative and quantitative methods for analysis of root exudates are poorly developed. In this study, Fraxinus mandshurica was used as research material for studying the influence of different collection times on the chemical composition of root exudate. The study employed qualitative and metabolomic analyses of root exudates with gas chromatography-mass spectrometry, and quantitatively analyzed root exudates of F. mandshurica with ultrahigh performance liquid chromatography (UPLC) and high performance ion chromatography (HPIC) methods. Root exudates were collected after harvest for 24 h at 6 h-intervals, and then analyzed. The types of sugars, amino acids, and organic acids in the root exudates were not significantly different among the four exudate samples. The results indicated that collection time strongly influenced the level of organic compounds in root exudates from F. mandshurica, with an optimal collection time of 12 h. In addition, quantitative analysis of root exudates using UPLC and HPIC had greater advantage, when compared with conventional analytical methods.
- Subjects
HIGH performance liquid chromatography; ASH (Tree); ORGANIC acids; SOIL ecology; CARBON cycle; METABOLOMICS; RHIZOSPHERE
- Publication
Applied Ecology & Environmental Research, 2019, Vol 17, Issue 4, p9529
- ISSN
1589-1623
- Publication type
Article
- DOI
10.15666/aeer/1704_95299545