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- Title
Demonstration of spontaneously dividing male fetal cells in maternal blood by negative magnetic cell sorting and fish.
- Authors
Zheng, Yun-Ling; Craigo, Sabrina D.; Price, Cathy M.; Bianchi, Diana W.
- Abstract
We investigated a case of massive feto-maternal bleeding by using negative magnetic cell sorting (MACS) and fluorescent in situ hybridization (FISH). A 37-year-old pregnant woman had an uncomplicated amniocentesis for advanced maternal age at 16 weeks' gestation. The fetal karyotype was 46, XY. At 19 weeks' gestation, she had a minor car accident and slight vaginal bleeding. A subsequent Kleihauer-Betke test showed a 140 ml feto-maternal haemorrhage. Serial sonographic examinations indicated a normal fetus and placenta. We performed FISH analysis on maternal peripheral blood at 25 weeks. Anti-CD45 and MACS were used to deplete maternal leucocytes, enriching the proportion of fetal nucleated erythrocytes present. The isolated cells were analysed by using dual-colour FISH with X and Y specific probes. Approximately 65 800 nucleated cells were obtained after MACS depletion. A total of 234 cells were analysed by FISH. The results revealed that 70 of the nucleated cells (30 per cent) were male with one X and one Y signal. Among these cells, six male metaphases were observed in spontaneously dividing cells.
- Publication
Prenatal Diagnosis, 1995, Vol 15, Issue 6, p573
- ISSN
0197-3851
- Publication type
Article
- DOI
10.1002/pd.1970150612