We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
LINE-1 methylation level and prognosis in pancreas cancer: pyrosequencing technology and literature review.
- Authors
Yamamura, Kensuke; Kosumi, Keisuke; Baba, Yoshifumi; Harada, Kazuto; Gao, Feng; Zhang, Xiaobo; Zhou, Lei; Kitano, Yuki; Arima, Kota; Kaida, Takayoshi; Takeyama, Hideaki; Higashi, Takaaki; Imai, Katsunori; Hashimoto, Daisuke; Chikamoto, Akira; Tan, Xiaodong; Baba, Hideo
- Abstract
Purpose: Global DNA hypomethylation plays an important role in genomic instability and carcinogenesis. The long interspersed nucleotide element-1 (LINE-1) methylation level is a good surrogate marker of the global DNA methylation level. Previously, we demonstrated a strong relationship between LINE-1 hypomethylation and poor prognosis in certain cancers. However, the relationship between the LINE-1 methylation level and the clinical outcome of pancreatic cancer (PC) remains unclear. Methods: We used a pyrosequencing assay to measure LINE-1 methylation levels in 126 samples of resected PC and evaluated the prognostic value of the LINE-1 methylation level. Results: LINE-1 methylation levels were significantly lower in PC tissues than in matched noncancerous pancreatic tissues ( p = 0.039, n = 36). The tumoral LINE-1 methylation range was 41.3-92.8 ( n = 126, mean 77.7, median 78.5, standard deviation 5.7). The LINE-1 methylation level was unrelated to clinical and pathological features. Moreover, LINE-1 hypomethylation was not significantly associated with overall survival, cancer specific survival, or disease-free survival (log-rank p = 0.30, p = 0.18 and p = 0.50, respectively). Conclusion: The LINE-1 methylation level appears not to be associated with poor prognosis in PC. The effect of the LINE-1 methylation level on the survival of PC patients needs to be confirmed in a larger-cohort study.
- Subjects
PANCREATIC cancer; METHYLATION; BIOMETHYLATION; STANDARD deviations; CANCER cells
- Publication
Surgery Today, 2017, Vol 47, Issue 12, p1450
- ISSN
0941-1291
- Publication type
Article
- DOI
10.1007/s00595-017-1539-1