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- Title
Identification of Trypanosoma cruzi sublineages by the simple method of single-stranded conformation DNA polymorphism (SSCP).
- Authors
Hiroo Higo; Sachio Miura; Takeshi Agatsuma; Tatsuyuki Mimori; Tetsuo Yanagi; Moritoshi Iwagami; A. de Arias; Vivian Matta; Kenji Hirayama; Tsutomu Takeuchi; Isao Tada; Kunisuke Himeno
- Abstract
Abstract??Fifty-eight stocks ofTrypanosoma cruzifrom Latin America were genetically characterized using the methods of the polymerase chain reaction (PCR) and the single-stranded conformation DNA polymorphism (SSCP) with four genes, mini-exon, 24S? rRNA, 18Sr RNA, cruzipain, and a RAPD fragment DNA region, P7?P8. All the isolates examined were assigned toT. cruziI or subgroups ofT. cruziII by these methods. From these results, the SSCP analysis, which was simple to perform and highly sensitive to sequence variation, seemed to be a good modality for characterizingT. cruzi, particularly for subgroups ofT. cruziII. However, in several isolates ofT. cruziII, the subgroups determined with the SSCP of 24S? rRNA were not consistent with those determined with other genes, the SSCP of 18S rRNA and cruzipain, and the PCR of P7?P8, possibly because of the occurrence of rare genetic exchanges or mutations or both in natural populations of this parasite. The SSCP patterns of 24S??rRNA and 18S rRNA were highly variable in theT. cruziI isolates; therefore, analyses using both genes are considered to be one possible method for the characterization of isolates withinT. cruziI.
- Subjects
TRYPANOSOMA cruzi; DNA polymerases; POLYMERASE chain reaction; NUCLEIC acids
- Publication
Parasitology Research, 2007, Vol 100, Issue 5, p1023
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-006-0376-8