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- Title
Heartwood extract from Dalbergia sissoo promotes fracture healing and its application in ovariectomy-induced osteoporotic rats.
- Authors
Karvande, Anirudha; Khedgikar, Vikram; Kushwaha, Priyanka; Ahmad, Naseer; Kothari, Priyanka; Verma, Ashwni; Kumar, Padam; Nagar, Geet Kumar; Mishra, Prabhat Ranjan; Maurya, Rakesh; Trivedi, Ritu
- Abstract
Objectives This study was undertaken to investigate the effects of a heartwood ethanolic extract ( HEE) made from the Dalbergia sissoo on facture healing and in the prevention of pathological bone loss resulting from estrogen deficiency in ovariectomized (Ovx) rats. Methods Heartwood ethanolic extract (250, 500 and 1000 mg/kg per day) was administered orally immediately next day after drill-hole injury and continued for 2 weeks. Ovx rats received HEE at same doses for 12 weeks and compared with 17-β estradiol (E2; 100 μg/kg for 5 days/week subcutaneously) group. Confocal imaging for fracture healing, micro-architecture of long bones, biomechanical strength, formation of mineralized nodule by bone marrow osteoprogenitor cells, bone turnover markers and gene expression were studied. One-way ANOVA was used to test significance. Key findings Heartwood ethanolic extract treatment promoted fracture healing, formation of new bone at the drill-hole site and stimulated osteogenic genes at callus region. HEE administration to the Ovx rats exhibited better micro-architectural parameters at various anatomical positions, better bone biomechanical strength and more osteoprogenitor cells in the bone marrow compared with Ovx + vehicle group. HEE exhibited no uterine estrogenicity. Conclusions Oral administration of HEE was found to promote fracture healing and exhibited osteoprotective effect by possibly stimulation of osteoblast function.
- Subjects
FRACTURE healing; HEARTWOOD; DALBERGIA sissoo; OVARIECTOMY; LABORATORY rats
- Publication
Journal of Pharmacy & Pharmacology, 2017, Vol 69, Issue 10, p1381
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/jphp.12764