We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Role of gastric mucus secretion, oxinitrergic system and sulfhydryl groups on the gastroprotection elicited by Polygala cyparissias ( Polygalaceae) in mice.
- Authors
Klein Júnior, Luiz Carlos; Santin, José Roberto; Lemos, Marivane; Silveira, Alessandro Conrado de Oliveira; Rocha, Jady Alice Rodrigues; Beber, Ana Paula; Wagner, Theodoro Marcel; Bresolin, Tania Mari Bellé; Bella Cruz, Alexandre; Cechinel Filho, Valdir; Faloni de Andrade, Sérgio
- Abstract
Objectives This study has aimed to assess the mechanisms of action for the gastroprotective effect of the acetone extract ( PCAE) and methanol fraction ( PCMF) of Polygala cyparissias, as well as to evaluate the activity of 1,3,6,8-tetrahydroxy-2,7-dimethoxyxanthone ( 1), 1,7-dihydroxy-2,3-dimethoxyxanthone ( 2) and astragalin ( 3). Methods Gastric secretion and mucus content were determined by pylorus ligation in mice. Nitric oxide ( NO) and sulfhydryl group participation were observed by the pretreatment of mice with L-NAME or NEM. Acute ulcer was induced by ethanol/ HCl and chronic ulcer by acetic acid. Anti- Helicobacter pylori activity was evaluated by the agar solid dilution assay. Key findings Neither PCAE nor PCMF had the ability to reduce H+ concentration. However, both of them enhanced mucus secretion. PCAE demonstrated its gastroprotection in a NO-dependent manner, while PCMF exerted the activity depending on the sulfhydryl group. In chronic ulcer, the curative ratios for the PCAE and PCMF were 67.5 and 58.4%, respectively. No effect over H. pylori was detected. Compounds 1, 2 and 3 were able to reduce lesions in the order of 79.6, 73.8 and 67.6%, respectively. Conclusions The data suggested that PCAE and PCMF displayed antiulcer activity due to different mechanisms and with the participation of phenolic compounds obtained from the plant.
- Subjects
ACETONE; POLYGALA; HELICOBACTER pylori; PHENOLS; AGAR
- Publication
Journal of Pharmacy & Pharmacology, 2013, Vol 65, Issue 5, p767
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/jphp.12038